Anti-Mov10抗体(ab80613)
Key features and details
- Rabbit polyclonal to Mov10
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Mov10抗体
参阅全部 Mov10 一抗 -
描述
兔多克隆抗体to Mov10 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa, 293T and NIH3T3 whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Antibody was affinity purified using an epitope specific to MOV10 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab80613于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 114 kDa.
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IP |
Use at 6 µg/mg of lysate.
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说明 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 114 kDa. |
IP
Use at 6 µg/mg of lysate. |
靶标
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相关性
MOV10 may be an helicase with an important function in development and/or control of cell proliferation. RNA silencing processes are guided by small RNAs known as siRNAs and microRNAs (miRNAs). They reside in ribonucleoprotein complexes, which guide the cleavage of complementary mRNAs or affect stability and translation of partial complementary mRNAs. Argonaute (Ago) proteins are at the heart of silencing effector complexes and bind the single-stranded siRNA and miRNA. Ago1- and Ago2-containing complexes have been purified from human cells, resulting in the discovery of novel factors such as the putative RNA helicase MOV10, and the RNA recognition motif (RRM)-containing protein TNRC6B/KIAA1093. The new proteins localize, similar to Ago proteins, to mRNA-degrading cytoplasmic P bodies, and they are functionally required to mediate miRNA-guided mRNA cleavage. -
细胞定位
Cytoplasmic -
数据库链接
- Entrez Gene: 457099 Chimpanzee
- Entrez Gene: 523206 Cow
- Entrez Gene: 483219 Dog
- Entrez Gene: 101128877 Gorilla
- Entrez Gene: 4343 Human
- Entrez Gene: 17454 Mouse
- Entrez Gene: 100458347 Orangutan
- Omim: 610742 Human
see all -
别名
- DKFZp667O1423 antibody
- FLJ32791 antibody
- fSAP113 antibody
see all
图片
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Lane 1 : Anti-Mov10 antibody (ab80613) at 1 µg/ml
Lanes 2-5 : Anti-Mov10 antibody (ab80613) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 114 kDa
Observed band size: 114 kDa
Exposure time: 3 minutes -
Immunoprecipitation of HeLa whole cell lysate using ab80613 at 6µg/mg (lane 1) or control IgG (lane 2). Samples were analysed by Western blot using ab80613 at 1µg/ml.
Detection: Chemiluminescence with an exposure time of 10 seconds.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab80613 被引用在 13 文献中.
- Wang L et al. MOV10 Helicase Interacts with Coronavirus Nucleocapsid Protein and Has Antiviral Activity. mBio 12:e0131621 (2021). PubMed: 34517762
- Yang H et al. LINC00667 promotes the proliferation, migration, and pathological angiogenesis in non-small cell lung cancer through stabilizing VEGFA by EIF4A3. Cell Biol Int 44:1671-1680 (2020). PubMed: 32281700
- Shah R et al. Single-cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection. J Vis Exp N/A:N/A (2020). PubMed: 33191939
- Liu D et al. Effect of P-body component Mov10 on HCV virus production and infectivity. FASEB J N/A:N/A (2020). PubMed: 32496609
- Liu T et al. The MOV10 helicase restricts hepatitis B virus replication by inhibiting viral reverse transcription. J Biol Chem 294:19804-19813 (2019). PubMed: 31722967