小鼠on小鼠Polymer IHC试剂盒(ab127055)

概述

  • 产品名称
    小鼠on小鼠Polymer IHC试剂盒
  • 样品类型
    Tissue
  • 种属反应性
    与反应: Mouse
  • 产品概述

    ab127055 is a robust ONE-step polymer system that provides increased sensitivity, time savings and detection simplicity. ab127055 allows the use of mouse antibodies on mouse tissue to obtain excellent signal intensities and clean background. The antibody-antigen interaction is visualized by adding a HRP conjugated secondary antibody followed by a chromogen (e.g. DAB (ab94665)) that yields a permanent brown precipitate. This system is also biotin-free, which eliminates background staining found with traditional biotin-based detection methods and improves signal-to-noise ratios.

  • 说明

    Additional materials required:

    • Antigen Retrieval Buffer (100X Citrate Buffer pH 6.0) (ab94674)
    • Antigen Retrieval Buffer (100X EDTA Buffer, pH 8.0) (ab94677)
    • Antigen Retrieval Buffer (100X Tris Buffer, pH 10.0) (ab94680)
    • Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab94681)
    • DAB substrate (ab94665)
  • 经测试应用
    适用于: IHC-Pmore details

性能

应用

Our Abpromise guarantee covers the use of ab127055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.

图片

  • Mouse Pan CK (Clone AE1/AE3) antibody staining Pan CK in mouse colon with the use of ab127055.
  • Immunohistochemical analysis with ab127055 showing Dazl and Boll protein expression during mouse fetal ovary development.

    High expression of Dazl (top panel) is seen in germ cells through all stages of development. Boll (bottom panel) is only expressed in germ cells during e15.5.

  • Immunohistochemical analysis of mouse eye using ab127055 to optimize signal to background staining ratio.

     

  • Mouse Pax-5 antibody staining Pax-5 in mouse spleen with the use of the ab127055.

实验方案

文献

This product has been referenced in:
  • Nie Y  et al. Palmdelphin promotes myoblast differentiation and muscle regeneration. Sci Rep 7:41608 (2017). Read more (PubMed: 28148961) »
  • Boutilier JK  et al. Gene Expression Networks in the Murine Pulmonary Myocardium Provide Insight into the Pathobiology of Atrial Fibrillation. G3 (Bethesda) 7:2999-3017 (2017). IHC-P ; Mouse . Read more (PubMed: 28720711) »

See all 8 Publications for this product

客户评价及客户问答

Mouse on Mouse Polymer IHC kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Tissue: paraffin embedded mouse xenograft tumor sections

Antigen retrieval: TE pH9, blocked with rodent block from kit.
Antibody diluent: 2% BSA
Primary antibody: mouse anti-Smooth muscle actin, 1:200, overnight incubation at 4oC
Secondary antibody: Mouse on mouse polymer HRP secondary antibody used at 2x dilution, incubate for 30 minutes at room temperature
Detected with DAB kit from Vector lab
Username

Abcam user community

Verified customer

提交于 Jan 03 2018

Life saving Mouse on Mouse Polymer IHC Kit for IHC

Excellent Excellent 5/5 (Ease of Use)
Abreviews
We have been having an issue getting this elastase staining to work o mouse lung tissue, given that the elastase ab (ab52484) is mouse originated. We've decided to order this kit and give it a try and did not regret ever since. It was a great kit that allows us to clearly visualized histone staining in addition to the other markers. In the following pictogram, elastase+ signal was labeled as red, while the brown cells are neutrophils.
Username

Jim Hsiao

Verified customer

提交于 Dec 19 2017

Postnatal day 6 mouse testes were fixed with 4% paraformaldehyde. Tissue was embedded in O.C.T. and frozen. 5 micron sections were cut and transferred to slides. Sections were permeabilized with 0.1% Triton X-100 in PBS, and blocked with a 50/50 mixture of Rodent Block and 3% BSA in 0.1% Triton X-100 + PBS. Sections were then incubated for 1h at RT with either (A) no primary antibody or (B) anti-DDX4 (Abcam ab27591) at a 1:100 dilution in a 50/50 mixture of Rodent Block and 3% BSA in 0.1% Triton X-100 + PBS. Sections were then washed 3X with 0.1% Triton X-100 in PBS. Donkey Anti-Mouse 555 (Abcam ab150110) applied at a 1:500 dilution in 3% BSA in 0.1% Triton X-100 + PBS. Sections were then mounted after washing 3X with 0.1% Triton X-100 in PBS.
Username

Mr. Bryan Niedenberger

Verified customer

提交于 Nov 21 2016

Thank you for your enquiry and your interest in our products.

I understand from your previous e-mail that your customer will perform IHC-P with samples from either rat or rabbit.

I can confirm that ab80436 EXPOSE Mouse and Rabbit Sp...

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Thank you for contacting us.

As requested, I have attached the datasheet for ab7032 as well as our Mouse on Mouse staining protocol. Abcam also carries a Mouse on Mouse staining kit which contains all of the reagents need to perform mouse ...

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Thank you for contacting us.

This kit will work with mouse IgM antiobodies as well.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoc...

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ab127055 kit can be used with any mouse antibody - this mean the kit would be suitable.

Please note ab49536 is tested and guaranteed to be with mouse species however ab57752 is only tested with human sampl...

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Thank you for contacting us.

I have found some information regarding the anti-IL-10 antibodies which we spoke of. To begin, I have found that Mouse IL-10 and Human IL-10 have only a 73% homology which is especially poor in the region around ...

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Vielen Dank für Ihre Anfrage.

Gerne senden wir Ihnen einen kostenlosen Ersatz. Bitte teilen Sie mir doch mit, wann (ungefähr) Sie den Antikörper gekauft haben, damit ich Ihre Bestellung heraus suchen kann.

Wenn Sie eine...

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Thank you for contacting us.

You had previously been in contact with my colleague heather regarding staining observed in your negative control using these mouse monoclonal Cytokeratin antibodies on mouse tissue. To clarify, is your negative c...

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1-10 of 18 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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