小鼠单克隆抗体[SB62a]抗兔IgG light chain (Alkaline Phosphatase) (ab99696)
Key features and details
- Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Mouse
- Isotype: IgG2b
- Suitable for: ELISA, WB
Related conjugates and formulations
概述
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产品名称
小鼠单克隆抗体[SB62a]抗兔IgG light chain (Alkaline Phosphatase)
参阅全部 IgG Light chain 二抗 -
宿主
Mouse -
靶标种属
Rabbit -
特异性
ab99696 reacts with the light chain of native Rabbit IgG primary antibodies and does not bind to the reduced and denatured Rabbit IgG heavy chain band (50 kD). -
经测试应用
适用于: ELISA, WBmore details -
免疫原
Full length protein corresponding to Rabbit IgG Light chain. Rabbit Light Chain
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偶联物
Alkaline Phosphatase
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 8.00
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.0095% Magnesium chloride, 0.605% Tris -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
SB62a -
同种型
IgG2b -
轻链类型
lambda -
研究领域
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab99696于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ELISA |
1/2000 - 1/4000.
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WB |
Use at an assay dependent concentration.
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说明 |
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ELISA
1/2000 - 1/4000. |
WB
Use at an assay dependent concentration. |
图片
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Western blot showing that Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (AP) (ab99696) specifically binds to light-chains
Left blot: Coomassie stain showing the rabbit heavy (~50kDa) and light-chains (~25 kDa)
Lane 1: 10 µg rabbit monoclonal was loaded
Lane 2: 5 µg rabbit monoclonal was loaded
Lane 3: 2 µg rabbit monoclonal was loadedRight blot: WB using Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (AP) (ab99696) as secondary antibody at 1/10,000
Lane 1: 1 µg rabbit monoclonal was loaded
Lane 2: 0.5 µg rabbit monoclonal was loaded
Lane 3: 0.25 µg rabbit monoclonal was loaded -
All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1 µg/ml
Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.
Secondary
Lanes 1 - 2 : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG - light chain (AP) (ab99696) at 1/5000 dilution
Lanes 3 - 4 : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG - light chain (AP) (ab99696) at 1/20000 dilution
Lanes 5 - 6 : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG - light chain (AP) (ab99696) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab99696 被引用在 2 文献中.
- Ao X et al. lincRNA-p21 inhibits the progression of non-small cell lung cancer via targeting miR-17-5p. Oncol Rep 41:789-800 (2019). PubMed: 30535441
- Castro-Severyn J et al. Arsenic Response of Three Altiplanic Exiguobacterium Strains With Different Tolerance Levels Against the Metalloid Species: A Proteomics Study. Front Microbiol 10:2161 (2019). PubMed: 31611848