小鼠Laminin ELISA试剂盒(ab119572)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 156 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠Laminin ELISA试剂盒
参阅全部 Laminin beta 2 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts -
检测类型
Sandwich (quantitative) -
灵敏度
< 10 pg/ml -
范围
156 pg/ml - 10000 pg/ml -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
Abcam’s mouse Laminin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse Laminin in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
A polyclonal antibody from rabbit specific for Laminin has been precoated onto 96-well plates. Standards(from murine sarcoma basement membrane) and test samples are added to the wells, a biotinylated detection polyclonal antibody from rabbit specific for Laminin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Laminin amount of sample captured in plate.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 标识符 1 x 96 tests 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml 1 x 12ml Anti-mouse Laminin Antibody Microplate (12 x 8 wells) 1 unit 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl 1 x 100µl Biotinylated anti-mouse Laminin antibody 1 x 100µl 1 x 100µl Lyophilized Recombinant mouse Laminin standard 2 x 10ng 2 x 10ng Plate Seal 1 x 4 units 1 x 4 units Sample Diluent Buffer Green Cap 1 x 30ml 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml 1 x 10ml Wash Buffer (25X) 1 x 20ml 1 x 20ml -
研究领域
- Information by UniProt
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别名
- Lamb2
- LAMB2_HUMAN
- Laminin B1s chain
see all -
数据库链接
- Entrez Gene: 16779 Mouse
- SwissProt: Q61292 Mouse
- Unigene: 27560 Mouse
- Unigene: 425599 Mouse
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab119572 被引用在 6 文献中.
- Li L et al. Ferroportin-dependent ferroptosis induced by ellagic acid retards liver fibrosis by impairing the SNARE complexes formation. Redox Biol 56:102435 (2022). PubMed: 36029649
- Santini-González J et al. In vitro generation of peri-islet basement membrane-like structures. Biomaterials 273:120808 (2021). PubMed: 33895491
- Li J et al. Melatonin attenuates renal fibrosis in diabetic mice by activating the AMPK/PGC1a signaling pathway and rescuing mitochondrial function. Mol Med Rep 19:1318-1330 (2019). PubMed: 30535482
- Fields B et al. The Adult Murine Intestine is Dependent on Constitutive Laminin-?1 Synthesis. Sci Rep 9:19303 (2019). PubMed: 31848396
- Barros D et al. Engineering hydrogels with affinity-bound laminin as 3D neural stem cell culture systems. Biomater Sci 7:5338-5349 (2019). PubMed: 31620727
- Hsu CC et al. Fabrication of Hemin-Doped Serum Albumin-Based Fibrous Scaffolds for Neural Tissue Engineering Applications. ACS Appl Mater Interfaces 10:5305-5317 (2018). ELISA . PubMed: 29381329