小鼠IP10 ELISA试剂盒(ab100675)
Key features and details
- Sensitivity: 15 pg/ml
- Range: 8.2 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠IP10 ELISA试剂盒
参阅全部 IP10 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 15 pg/ml -
范围
8.2 pg/ml - 2000 pg/ml -
回收率
97 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 98.86 88% - 107% Serum 97.68 86% - 106% Plasma 96.42 85% - 104% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
Abcam’s IP10 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IP10 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse IP10 coated on a 96- well plate. Standards and samples are pipetted into the wells and IP10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IP10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IP10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Mouse IP-10 ELISA Kit (CXCL10) (ab214563) from our SimpleStep ELISA® range.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 80x HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Mouse IP10 2 vials IP10 Microplate (12 x 8 wells) 1 unit Recombinant Mouse IP10 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3. -
序列相似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻译后修饰
CXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes. -
细胞定位
Secreted. - Information by UniProt
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别名
- Interferon gamma induced factor MOB1, mouse, homolog of
- Interferon gamma induced protein 10
- 10 kDa interferon gamma induced protein
see all -
数据库链接
- Entrez Gene: 15945 Mouse
- SwissProt: P17515 Mouse
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab100675 被引用在 1 文献中.
- Zaheer S et al. Clinical course of myelin oligodendrocyte glycoprotein 35-55 induced experimental autoimmune encephalomyelitis is aggravated by glia maturation factor. Neurochem Int 60:215-9 (2012). PubMed: 22226840