小鼠IL-10 ELISA试剂盒 (without plates) (ab47599)
概述
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产品名称
小鼠IL-10 ELISA试剂盒 (without plates)
参阅全部 IL-10 试剂盒 -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 20 pg/ml -
范围
31.25 pg/ml - 1000 pg/ml -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
Abcam’s IL-10 Murine in vitro ELISA Set is designed for the quantitative measurement of Murine IL-10 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
The murine IL-10 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
This ELISA Set is intended to measure murine IL-10 in cell -culture supernatants, as well as in serum samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
Recognizes both natural and recombinant Murine IL-10
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Reagents
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 5 x 96 tests 15 x 96 tests 10 x 96 tests Chromogen TMB Substrate Solution 2 vials 6 vials 4 vials Detection biotinylated anti-murine IL-10 antibody 1 x 0.55ml 3 x 0.55ml 2 x 0.55ml Murine IL-10 Capture antibody 1 x 0.5ml 3 x 0.5ml 2 x 0.5ml Murine IL-10 standard 5 vials 15 vials 10 vials Streptavidin-HRP 1 vial 3 vials 2 vials -
研究领域
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功能
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
组织特异性
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
序列相似性
Belongs to the IL-10 family. -
细胞定位
Secreted. - Information by UniProt
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别名
- CSIF
- Cytokine synthesis inhibitory factor
- IL 10
see all -
数据库链接
- Entrez Gene: 16153 Mouse
- SwissProt: Q0VBJ1 Mouse
- SwissProt: P18893 Mouse
- Unigene: 874 Mouse
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab47599于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Sandwich ELISA |
Use at an assay dependent concentration.
|
说明 |
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Sandwich ELISA
Use at an assay dependent concentration. |
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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