The standard curve was as described as the typical curve. One concentration had to be eliminated to give a high R^2 value.
Samples were from rat (normal out-bred sprague dawley from Harlan) liver tissue, homogenized using a 1% np-40 buffer. Although a 1:10 dilution was used for the samples (which was sufficiently in range of the IL1b ELISA (ab100768) standards), the absorbances were out of range of the standards.
For the samples that did fall in the standard range, the levels of IL6 in control rats seem to be a little higher (~225-325pg/ml) than the expected range (~125-200pg/ml).
Samples were from rat (normal out-bred sprague dawley from Harlan) liver tissue, homogenized using a 1% np-40 buffer. Although a 1:10 dilution was used for the samples (which was sufficiently in range of the IL1b ELISA (ab100768) standards), the absorbances were out of range of the standards.
For the samples that did fall in the standard range, the levels of IL6 in control rats seem to be a little higher (~225-325pg/ml) than the expected range (~125-200pg/ml).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Apr 02 2014