小鼠IL-6 ELISA试剂盒(ab100712)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 0.82 pg/ml - 600 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠IL-6 ELISA试剂盒
参阅全部 IL-6 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 2 pg/ml -
范围
0.82 pg/ml - 600 pg/ml -
回收率
94 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 96.18 86% - 103% Serum 93.39 83% - 103% Plasma 94.53 84% - 104% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse
预测可用于: Human -
产品概述
Mouse IL-6 ELISA kit is designed for the quantitative measurement of Mouse IL-6 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse IL-6 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IL-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Biotinylated anti-Mouse IL-6 2 vials IL-6 Microplate (12 x 8 wells) 1 unit Recombinant mouse IL-6 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. -
疾病相关
Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. -
序列相似性
Belongs to the IL-6 superfamily. -
翻译后修饰
N- and O-glycosylated. -
细胞定位
Secreted. - Information by UniProt
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别名
- Interleukin BSF 2
- B cell differentiation factor
- B cell stimulatory factor 2
see all -
数据库链接
- Entrez Gene: 3569 Human
- Entrez Gene: 16193 Mouse
- Omim: 147620 Human
- SwissProt: P05231 Human
- SwissProt: P08505 Mouse
- Unigene: 654458 Human
- Unigene: 1019 Mouse
图片
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Standard curve of msIL-6 in different diluents with background signal subtracted (duplicates; +/- SD).
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IL-6 measured in mouse tissue lysates (diluted in the range of 1:20 to 1:200; expressed as per mg of extracted protein), with background signal subtracted (duplicates; +/- SD).
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msIL-6 measured in biological fluids, background signal subtracted (duplicates +/- SD).
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IL-6 detected in supernatants from RAW 246.7 control cells (C) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) (P), or 24 hours with PMA and 1 ug x mL-1 of LPS (Sigma) (P+L) for the last 6 hours. Results shown after background signal was subtracted (duplicates +/- SD).
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Representative standard curve using ab100712
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Representative standard curve using ab100712
数据表及文件
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SDS download
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Datasheet download
文献 (124)
ab100712 被引用在 124 文献中.
- Szlasa W et al. Betulin and Its Derivatives Reduce Inflammation and COX-2 Activity in Macrophages. Inflammation 46:573-583 (2023). PubMed: 36282372
- Ferrer-Pérez C et al. Effect of Voluntary Wheel-Running Exercise on the Endocrine and Inflammatory Response to Social Stress: Conditioned Rewarding Effects of Cocaine. Biomedicines 10:N/A (2022). PubMed: 36289635
- Qu C et al. Nano-Honokiol ameliorates the cognitive deficits in TgCRND8 mice of Alzheimer's disease via inhibiting neuropathology and modulating gut microbiota. J Adv Res 35:231-243 (2022). PubMed: 35024199
- Liu J et al. Study on Toll-Like Receptor 2-Mediated Inflammation-Induced Familial Hypertension Combined with Hyperlipemia and Its Mechanism. J Healthc Eng 2022:1473597 (2022). PubMed: 35035808
- Ponzetti M et al. Lipocalin 2 Influences Bone and Muscle Phenotype in the MDX Mouse Model of Duchenne Muscular Dystrophy. Int J Mol Sci 23:N/A (2022). PubMed: 35055145