小鼠IL-17 ELISA试剂盒(ab100702)
Key features and details
- Sensitivity: 6 pg/ml
- Range: 6.1 pg/ml - 600 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠IL-17 ELISA试剂盒
参阅全部 IL-17A 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 6 pg/ml -
范围
6.1 pg/ml - 600 pg/ml -
回收率
99 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 99.62 85% - 105% Serum 101.38 87% - 107% Plasma 97.47 84% - 103% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
Abcam’s IL-17 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-17 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse IL-17 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-17 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-17 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-17 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 700X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Mouse IL-17 2 vials IL-17 Microplate (12 x 8 wells) 1 unit Recombinant Mouse IL-17 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts. -
组织特异性
Restricted to activated memory T-cells. -
序列相似性
Belongs to the IL-17 family. -
翻译后修饰
Found both in glycosylated and nonglycosylated forms. -
细胞定位
Secreted. - Information by UniProt
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别名
- CTLA 8
- CTLA-8
- CTLA8
see all -
数据库链接
- Entrez Gene: 16171 Mouse
- SwissProt: Q62386 Mouse
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab100702 被引用在 16 文献中.
- Xiang J et al. Lidocaine relieves murine allergic rhinitis by regulating the NF-κB and p38 MAPK pathways. Exp Ther Med 23:193 (2022). PubMed: 35126696
- Yao Q et al. 2'-Fucosyllactose Ameliorates Inflammatory Bowel Disease by Modulating Gut Microbiota and Promoting MUC2 Expression. Front Nutr 9:822020 (2022). PubMed: 35252301
- Fu Y et al. Fingolimod protects against experimental necrotizing enterocolitis by regulating intestinal T cell differentiation. Transl Pediatr 11:575-588 (2022). PubMed: 35558966
- AlEraky DM et al. Potential Antigenic Candidates for the Development of Peptide-Based Vaccines to Induce Immunization against Helicobacter pylori Infection in BALB/c Mice. Int J Mol Sci 23:N/A (2022). PubMed: 36361614
- He Q et al. Triptolide Inhibits Th17 Response by Upregulating microRNA-204-5p and Suppressing STAT3 Phosphorylation in Psoriasis. Genet Res (Camb) 2022:7468396 (2022). PubMed: 36474621