小鼠IL-12 p70 ELISA试剂盒(ab119531)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 15.6 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠IL-12 p70 ELISA试剂盒
参阅全部 IL-12 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 7 8.3% 批次间 样品 n Mean SD CV% Overall 7 11% -
样品类型
Cell culture supernatant, Serum -
检测类型
Sandwich (quantitative) -
灵敏度
10 pg/ml -
范围
15.6 pg/ml - 1000 pg/ml -
回收率
106 %
特定样本回收率 样品类型 平均% 范围 Serum 106 95% - 118% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
IL-20 p70 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-20 p70 in serum and cell culture supernatants.
IL-12 p70 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with a Biotin-conjugated anti-mouse IL-12 p70 detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and then unbound conjugates are washed away using wash buffer. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-12 p70 captured on the plate.
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说明
The standard is affinity purified.
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 20X Assay Buffer Concentrate 1 x 5ml 20X Wash Buffer Concentrate 1 x 50ml Adhesive Films 4 units Biotin-Conjugate anti-mouse IL-12 p70 monoclonal antibody 1 x 70µl Microplate coated with anti-mouse IL-12 p70 monoclonal antibody (12 x 8 wells) 1 unit Mouse IL-12 p70 Standard, lyophilized (2 ng/mL on reconstitution). 2 vials Sample Diluent 1 x 12ml Stop Solution (1M Phosphoric acid) 1 x 15ml Streptavidin-HRP 1 x 150µl TMB Substrate Solution 1 x 15ml -
研究领域
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功能
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. -
序列相似性
Belongs to the IL-6 superfamily. -
细胞定位
Secreted. - Information by UniProt
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别名
- CLMF
- CLMF p35
- CLMF p40
see all -
数据库链接
- Entrez Gene: 16159 Mouse
- Entrez Gene: 16160 Mouse
- SwissProt: P43431 Mouse
- SwissProt: P43432 Mouse
- Unigene: 103783 Mouse
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab119531 被引用在 5 文献中.
- Lo YT et al. Immunogenicity of a secreted, C-terminally truncated, form of bovine viral diarrhea virus E2 glycoprotein as a potential candidate in subunit vaccine development. Sci Rep 13:296 (2023). PubMed: 36609424
- Chen HQ et al. Effects of Astragalus membranaceus on systemic lupus erythematosus in a mouse model of pregnancy. Immun Inflamm Dis 10:e624 (2022). PubMed: 35634952
- Xiu W et al. Potentiating hypoxic microenvironment for antibiotic activation by photodynamic therapy to combat bacterial biofilm infections. Nat Commun 13:3875 (2022). PubMed: 35790729
- Nath A et al. Gold-manganese oxide nanocomposite suppresses hypoxia and augments pro-inflammatory cytokines in tumor associated macrophages. Int Immunopharmacol 57:157-164 (2018). PubMed: 29499453
- Pan Y et al. Polyphenols in Liubao Tea Can Prevent CCl4-Induced Hepatic Damage in Mice through Its Antioxidant Capacities. Nutrients 10:N/A (2018). PubMed: 30201943