小鼠Galectin 1 ELISA试剂盒(ab119595)
Key features and details
- Sensitivity: 5 pg/ml
- Range: 156 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠Galectin 1 ELISA试剂盒
参阅全部 Galectin 1 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% 1 16 343pg/ml 22.29 = 6.5% 2 16 1747pg/ml 132.77 = 7.6% 3 16 4774pg/ml 300.76 = 8.5% 批次间 样品 n Mean SD CV% 1 24 318pg/ml 20.67 = 6.5% 2 24 1697pg/ml 144.24 = 8.5% 3 24 4684pg/ml 304.46 = 6.5% -
样品类型
Cell culture supernatant, Serum, Plasma, Cell Lysate -
检测类型
Sandwich (quantitative) -
灵敏度
< 5 pg/ml -
范围
156 pg/ml - 10000 pg/ml -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
Abcam’s mouse Galectin 1 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse Galectin 1 in cell culture supernatants, serum and plasma (heparin, EDTA).
A Galectin 1 specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for Galectin 1 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse Galectin 1 amount of sample captured in plate.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 标识符 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml Anti-mouse Galectin 1 antibody Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti-mouse Galectin-1 antibody 1 x 100µl Lyophilized recombinant mouse Galectin 1 standard 2 x 10ng Plate Seal 4 units Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
研究领域
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功能
May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. -
组织特异性
Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells. -
序列相似性
Contains 1 galectin domain. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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别名
- 14 kDa laminin-binding protein
- 14 kDa lectin
- Beta galactoside binding lectin
see all -
数据库链接
- Entrez Gene: 16852 Mouse
- SwissProt: P16045 Mouse
- Unigene: 43831 Mouse
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab119595 被引用在 3 文献中.
- Losada-Barragán M et al. Thymic Microenvironment Is Modified by Malnutrition and Leishmania infantum Infection. Front Cell Infect Microbiol 9:252 (2019). PubMed: 31355153
- Hartman ES et al. Increased Reactive Oxygen Species and Cell Cycle Defects Contribute to Anemia in the RASA3 Mutant Mouse Model scat. Front Physiol 9:689 (2018). PubMed: 29922180
- Kanda A et al. Advanced glycation endproducts link inflammatory cues to upregulation of galectin-1 in diabetic retinopathy. Sci Rep 7:16168 (2017). Functional Studies ; Mouse . PubMed: 29170525