小鼠EGF ELISA试剂盒(ab100679)
Key features and details
- Sensitivity: 3 pg/ml
- Range: 3.28 pg/ml - 800 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠EGF ELISA试剂盒
参阅全部 EGF 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 3 pg/ml -
范围
3.28 pg/ml - 800 pg/ml -
回收率
96 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 112.4 83% - 107% Serum 88.33 75% - 101% Plasma 90.23 80% - 101% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
Abcam’s EGF (Epidermal growth factor) Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse EGF in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse EGF coated on a 96- well plate. Standards and samples are pipetted into the wells and EGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse EGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of EGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse EGF 2 vials EGF Microplate (12 x 8 wells) 1 unit Recombinant Mouse EGF Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941). -
组织特异性
Expressed in kidney, salivary gland, cerebrum and prostate. -
疾病相关
Hypomagnesemia 4 -
序列相似性
Contains 9 EGF-like domains.
Contains 9 LDL-receptor class B repeats. -
翻译后修饰
O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor). -
细胞定位
Membrane. - Information by UniProt
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别名
- Beta urogastrone
- beta-urogastrone
- EGF
see all -
数据库链接
- Entrez Gene: 13645 Mouse
- SwissProt: P01132 Mouse
- Unigene: 252481 Mouse
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab100679 被引用在 5 文献中.
- Onal S et al. Breast cancer cells and macrophages in a paracrine-juxtacrine loop. Biomaterials 267:120412 (2021). PubMed: 33161320
- Li J et al. HOXC10 promotes cell migration, invasion, and tumor growth in gastric carcinoma cells through upregulating proinflammatory cytokines. J Cell Physiol 235:3579-3591 (2020). PubMed: 31552684
- Abughanam G et al. Mesenchymal Stem Cells Extract (MSCsE)-Based Therapy Alleviates Xerostomia and Keratoconjunctivitis Sicca in Sjogren's Syndrome-Like Disease. Int J Mol Sci 20:N/A (2019). PubMed: 31557796
- Sarnelli G et al. Palmitoylethanolamide Modulates Inflammation-Associated Vascular Endothelial Growth Factor (VEGF) Signaling via the Akt/mTOR Pathway in a Selective Peroxisome Proliferator-Activated Receptor Alpha (PPAR-a)-Dependent Manner. PLoS One 11:e0156198 (2016). PubMed: 27219328
- Good M et al. Amniotic fluid inhibits Toll-like receptor 4 signaling in the fetal and neonatal intestinal epithelium. Proc Natl Acad Sci U S A 109:11330-5 (2012). Mouse . PubMed: 22733781