Application
Western blot
Sample
Pig Tissue lysate - other (Mitochondria)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
20 µg
Specification
Mitochondria
Blocking step
Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/5000
Secondary antibody
Dilution
1/15000
Name
Non-Abcam antibody was used: Donkey anti-rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
NIR
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 80 kDa Non-specific: 65 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Dec 19 2016