Mitochondrial Staining试剂盒- NIR Fluorescence - Cytopainter (ab176747)
概述
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产品名称
Mitochondrial Staining试剂盒- NIR Fluorescence - Cytopainter
参阅全部 Mitochondria 试剂盒 -
样品类型
Adherent cells, Suspension cells -
检测类型
Cell-based (qualitative) -
种属反应性
与反应: Mammals, Other species -
产品概述
Mitochondrial Staining Kit - NIR Fluorescence | Cytopainter (ab176747) is designed to label mitochondria in live cells with red fluorescence. The kit uses our proprietary dye that selectively accumulates in mitochondria probably via the mitochondrial membrane potential gradient. The red fluorescent mitochondrial stain used in the kit has Ex/Em = 640/660 nm (Cy5 filter-compatible). The mitochondrial indicator, a hydrophobic compound, easily permeates intact live cells and trapped in mitochondria after it gets into cells. This fluorescent mitochondrial indicator is retained in mitochondria for a long time since it carries a cell-retaining group. This key feature significantly increases the staining efficiency.
The kit can be readily adapted for many different types of fluorescence platforms, such as microplate assays, immunocytochemistry and flow cytometry. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components and can be used for both proliferating and non-proliferating cells.
Abcam's fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria, nuclei, etc. The selective labeling of live cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.
Review other dyes and kits for mitochondrial staining, or the live cell staining fluorescent dyes guide
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说明
Mitochondria are membrane-enclosed organelles found in most eukaryotic cells. Mitochondria are sometimes described as "cellular power plants" because they generate most of the cellular supply of ATP. In addition to supplying cellular energy, mitochondria are involved in a range of other processes, such as signalling, cellular differentiation, cell death, as well as the control of the cell cycle and cell growth. Mitochondria have been implicated in several human diseases, including mitochondrial disorders and cardiac dysfunction, and may play a role in the aging process. Although most of a cellular DNA is contained in the cell nucleus, the mitochondrion has its own independent genome.
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平台
Fluorescence microscope
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 500 tests Live Cell Staining Buffer 1 x 50ml MitoNIR Indicator 1 x 100µl -
研究领域
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图片
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab176747 被引用在 3 文献中.
- Miki K et al. ERR? enhances cardiac maturation with T-tubule formation in human iPSC-derived cardiomyocytes. Nat Commun 12:3596 (2021). PubMed: 34155205
- Tan JJ et al. Human iPS-derived pre-epicardial cells direct cardiomyocyte aggregation expansion and organization in vitro. Nat Commun 12:4997 (2021). PubMed: 34404774
- Ben Safta T et al. Granzyme B-activated p53 interacts with Bcl-2 to promote cytotoxic lymphocyte-mediated apoptosis. J Immunol 194:418-28 (2015). ICC/IF . PubMed: 25404359