MitoTox™ Complex I OXPHOS Activity Assay试剂盒(ab109903)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 5 hr
- Sample type: Inhibitor compounds
概述
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产品名称
MitoTox™ Complex I OXPHOS Activity Assay试剂盒
参阅全部 Complex I 试剂盒 -
检测方法
Colorimetric -
样品类型
Inhibitor compounds -
检测类型
Quantitative -
检测时间
5h 00m -
产品概述
MitoTox™ Complex I OXPHOS Activity Assay (ab109903) is designed for testing the direct inhibitory effect of compounds on Complex I activity in only 5 hours. Complex I extracted from the provided bovine heart mitochondria (a rich source of Complex I) is immunocaptured by specific antibodies on the plate. Complex I activity can be observed as decrease in absorbance at OD 340 nm. The intra-assay and inter-assay variation of this assay are both < 10%.
Inhibitory effects of compounds on Complex I activity can be tested in two different ways: 1. Screening format, where up to 23 compounds can be tested at a single concentration in triplicate; 2. Dose response (IC50) format, where two compounds known to affect Complex I activity can be tested at 11 different data points in triplicate.
Testing for mitochondrial function has become a key aspect of drug discovery. Mitochondria can be affected by drug treatment, resulting into cardio- and hepatotoxic side effects that can lead to drug withdrawal from the market. Therefore, there is increasing emphasis on testing the impact on mitochondria early on in the drug development process to reduce failure rates during preclinical and clinical phases.
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说明
Store Phospholipids, Bovine heart mitochondria, Ubiquinone and Activity Buffer at -80°C. Store all other components at 4°C.
Related products
Review the mitochondrial assay guide, or the full metabolism assay guide to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.
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平台
Microplate reader
性能
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存放说明
Please refer to protocols. -
组件 96 tests 12-channel reagent reservoirs 2 units 1X Mito Buffer 1 x 5ml 20X Wash Buffer 1 x 5ml Bovine heart mitochondria 1 x 360µl Complex I Activity Buffer 1 x 24ml Detergent 1 x 100µl Phospholipids 1 x 6ml Pre-coated 96-well microplate 1 unit Ubiquinone 1 1 x 60µl -
研究领域
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别名
- NADH dehydrogenase
图片
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Typical dose response curve for rotenone. Assay was performed following the Dose Response Assay Procedure using rotenone, a well known Complex I inhibitor. Rotenone was prepared in DMSO to generate a 10 mM stock. Starting with a 50 µM final concentration in well, 1:10 serial dilutions of rotenone were generated.
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab109903 被引用在 13 文献中.
- Zappelli E et al. α-Glyceryl-phosphoryl-ethanolamine protects human hippocampal neurons from ageing-induced cellular alterations. Eur J Neurosci 56:4514-4528 (2022). PubMed: 35902984
- Papachristodoulou A et al. NKX3.1 Localization to Mitochondria Suppresses Prostate Cancer Initiation. Cancer Discov 11:2316-2333 (2021). PubMed: 33893149
- Zhang X et al. ITGB2-mediated metabolic switch in CAFs promotes OSCC proliferation by oxidation of NADH in mitochondrial oxidative phosphorylation system. Theranostics 10:12044-12059 (2020). PubMed: 33204328
- Park SY & Koh HC FUNDC1 regulates receptor-mediated mitophagy independently of the PINK1/Parkin-dependent pathway in rotenone-treated SH-SY5Y cells. Food Chem Toxicol 137:111163 (2020). PubMed: 32001317
- Wang Y et al. Metformin Improves Mitochondrial Respiratory Activity through Activation of AMPK. Cell Rep 29:1511-1523.e5 (2019). PubMed: 31693892