Application
Western blot
Sample
Dog Tissue lysate - whole (left ventricle)
Loading amount
90 µg
Specification
left ventricle
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/250
Incubation time
17 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% milk in TTBS
Secondary antibody
Name
Non-Abcam antibody was used: HRP linked sheep anti-mouse IgG
Host species: Sheep
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 110 kDa
Additional data
Additional Notes
Left ventricle homogenate from 4 separate dogs were electrophoreses in 4-20% PAGE and transferred to Imobilon P membrane. Blots were developed by ECL method using HRP linked anti-mouse IgG (from Sheep).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. sudhish mishra
Verified customer
提交于 Jun 19 2007