Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (10% gel, at RT)
Sample
Hamster Cell lysate - whole cell (CHO cella)
Specification
CHO cella
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking buffer
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: anti rabbit IgG HRP linked antibody
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Bands
Specific: 110 kDa
Exposure
1 minute(s) and 0 second(s)
Positive control
imcd-3 Mouse cell line+ Transfection with MIB fused to GFPin CHO Cells
Additional data
Additional Notes
Discount code is 86098
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Miss. Bassma Khamaisi
Verified customer
提交于 Feb 04 2015