Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
Sample
Mouse Tissue sections (e11.5 embryo transverse cryosection of trunk)
Specification
e11.5 embryo transverse cryosection of trunk
Permeabilization
Yes - 1% saponin
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking solution
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: anti-mouse IgG (H+L) Alexa Fluor 647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 647
Dilution
1/500
Additional data
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Mar 24 2015