Anti-Metabotropic Glutamate Receptor 5抗体(ab53090)

概述

  • 产品名称
    Anti-Metabotropic Glutamate Receptor 5抗体
    参阅全部 Metabotropic Glutamate Receptor 5 一抗
  • 描述
    兔多克隆抗体to Metabotropic Glutamate Receptor 5
  • 特异性
    ab53090 detects endogenous levels of total Metabotropic Glutamate Receptor 5 protein.
  • 经测试应用
    适用于: IHC-Fr, ICC/IF, IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Chicken, Human
  • 免疫原

    N-terminal synthetic peptide derived from human Metabotropic Glutamate Receptor 5.

  • 阳性对照
    • WB: Mouse brain extracts IHC-P: Human brain tissue

性能

应用

Our Abpromise guarantee covers the use of ab53090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration. PubMed: 22962568
ICC/IF 1/100. PubMed: 19364772
IHC-P 1/50 - 1/100.
ELISA 1/5000.
WB 1/300 - 1/1000. Predicted molecular weight: 132 kDa.

靶标

  • 功能
    G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity.
  • 序列相似性
    Belongs to the G-protein coupled receptor 3 family.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Glutamate receptor metabotropic 5 antibody
    • GPRC1E antibody
    • Grm5 antibody
    • GRM5_HUMAN antibody
    • Metabotropic glutamate receptor 5 antibody
    • Metabotropic glutamate receptor 5 variant F antibody
    • Metabotropic glutamate receptor 5 variant G antibody
    • Metabotropic glutamate receptor 5 variant H antibody
    • mGlu5 antibody
    • mGluR5 antibody
    • PPP1R86 antibody
    • Protein phosphatase 1 regulatory subunit 86 antibody
    see all

图片

  • ab53090 staining Metabotropic Glutamate Receptor 5 (red) in rat hippocampla neuron by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 5% serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/200) for 24 hours at 22°C. An Alexa Fluor® 594-conjugated chicken anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Cyan - pre-synaptic marker Bassoon.

    See Abreview

  • All lanes : Anti-Metabotropic Glutamate Receptor 5 antibody (ab53090) at 1/300 dilution

    Lane 1 : Mouse brain extracts
    Lane 2 : Mouse brain extracts with blocking peptide


    Predicted band size : 132 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin embedded human brain tissue using ab53090 at 1/50 dilution, in the presence (right) and absence (left) of blocking peptide.
  • ab53090 staining Metabotropic Glutamate Receptor 5 in rat brain tissue sections by Immunohistochemistry (Free Floating).
    Animals were perfused fixed with 4% paraformaldehyde with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using ab53090 at a 1/300 dilution. The secondary used was an Alexa Fluor 488 conjugated goat anti-rabbit used at a 1/1000 dilution.ab53090 staining Metabotropic Glutamate Receptor 5 in rat brain tissue sections by Immunohistochemistry (Free Floating).

    Animals were perfused fixed with 4% paraformaldehyde with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using ab53090 at a 1/300 dilution. The secondary used was an Alexa Fluor 488 conjugated goat anti-rabbit used at a 1/1000 dilution.

    See Abreview

文献

This product has been referenced in:
  • Dai J  et al. Improved retinal function in RCS rats after suppressing the over-activation of mGluR5. Sci Rep 7:3546 (2017). Read more (PubMed: 28615682) »
  • Rossignol R  et al. Visual sensorial impairments in neurodevelopmental disorders: evidence for a retinal phenotype in fragile x syndrome. PLoS One 9:e105996 (2014). IHC-Fr ; Mouse . Read more (PubMed: 25153086) »

See all 9 Publications for this product

客户评价及客户问答

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 22°C
Sample
Rat Cell (rat hippocampal neuron)
Specification
rat hippocampal neuron
Permeabilization
Yes - 0.2%Triton
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 07 2014

Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (COS7)
Permeabilization
Yes - 0.2%Triton
Specification
COS7
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 07 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Commercial Peroxidase blocking buffer as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Commercial High pH Buffer
Sample
Human Tissue sections (Tendon)
Specification
Tendon
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. Sarah Franklin

Verified customer

提交于 Aug 14 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Aug 11 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Rat Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Aug 11 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Aug 11 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Sample
Rat Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.1% triton
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample
Mouse Tissue sections (vestibular nuclar)
Specification
vestibular nuclar
Permeabilization
Yes - 0.1% triton
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample
Chicken Tissue sections (vestibular neuclar)
Specification
vestibular neuclar
Permeabilization
Yes - 0.3% triton
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 17 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample
Rat Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.5% triton
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 17 2013

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