重组Anti-Metabotropic Glutamate Receptor 5抗体[EPR2425Y] - Low endotoxin,Azide free (ab219374)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2425Y] to Metabotropic Glutamate Receptor 5 - Low endotoxin, Azide free
- Suitable for: WB, Electron Microscopy, Flow Cyt (Intra), IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Metabotropic Glutamate Receptor 5抗体[EPR2425Y] - Low endotoxin,Azide free
参阅全部 Metabotropic Glutamate Receptor 5 一抗 -
描述
兔单克隆抗体[EPR2425Y] to Metabotropic Glutamate Receptor 5 - Low endotoxin,Azide free -
宿主
Rabbit -
特异性
The Human species recommendation is based on the WB results. This antibody has been tested for IHC-P and IHC-Fr in Human samples and we obtain positive signal only in IHC-Fr. We do not recommend this antibody for IHC-P in Human samples.
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经测试应用
适用于: WB, Electron Microscopy, Flow Cyt (Intra), IHC-P, IHC-Frmore details
不适用于: IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC: mouse brain lysate, rat and mouse cerebrum tissues. Flow Cyt (intra): SH-SY5Y cells
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常规说明
ab219374 is the carrier-free version of ab76316.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.20
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2425Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- HRP Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab196482)
- Alexa Fluor® 647 Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab196929)
- Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - BSA and Azide free (ab256589)
- APC Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab310880)
- PE Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab310939)
- Alexa Fluor® 488 Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab310960)
- Alexa Fluor® 594 Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab311666)
- Alexa Fluor® 568 Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab312940)
- Alexa Fluor® 555 Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab313151)
- Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab219374于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration.
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Electron Microscopy |
Use at an assay dependent concentration.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab199376- Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Use of HRP-conjugated or polymerized HRP secondary antibodies, stronger signals have been found using the polymerized HRP secondary. |
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IHC-Fr |
Use at an assay dependent concentration.
This antibody has been tested for IHC-P and IHC-Fr in Human samples and we obtain positive signal only in IHC-Fr. We do not recommend this antibody for IHC-P in Human samples. |
说明 |
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WB
Use at an assay dependent concentration. |
Electron Microscopy
Use at an assay dependent concentration. |
Flow Cyt (Intra)
Use at an assay dependent concentration. ab199376- Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Use of HRP-conjugated or polymerized HRP secondary antibodies, stronger signals have been found using the polymerized HRP secondary. |
IHC-Fr
Use at an assay dependent concentration. This antibody has been tested for IHC-P and IHC-Fr in Human samples and we obtain positive signal only in IHC-Fr. We do not recommend this antibody for IHC-P in Human samples. |
靶标
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功能
G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity. -
序列相似性
Belongs to the G-protein coupled receptor 3 family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2915 Human
- Entrez Gene: 108071 Mouse
- Entrez Gene: 24418 Rat
- Omim: 604102 Human
- SwissProt: P41594 Human
- SwissProt: Q3UVX5 Mouse
- SwissProt: P31424 Rat
- Unigene: 147361 Human
see all -
别名
- Glutamate receptor metabotropic 5 antibody
- GPRC1E antibody
- Grm5 antibody
see all
图片
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This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76316)
Immunohistochemical analysis of Paraffin-embedded rat cerebrum tissue sections labeling Metabotropic Glutamate Receptor 5 with ab76316 at 1/400 dilution followed by Goat Anti-Rabbit IgG H&L (HRP Polymer) secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).
Positive staining on rat cerebrum.
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This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76316)Immunohistochemical analysis of Paraffin-embedded mouse cerebrum tissue sections labeling Metabotropic Glutamate Receptor 5 with ab76316 at 1/400 dilution followed by Goat Anti-Rabbit IgG H&L (HRP Polymer) secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).Positive staining on mouse cerebrum.
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This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76316)
ab76316 staining Metabotropic Glutmate Receptor 5 in mouse caudate putamen/ Corpus callosum by immunohistochemistry. Tissue was fixed with formaldehyde and citrate-mediated antigen retrieval was performed. Samples were blocked with 1% BSA for 10 minutes at 21°C, before incubation with the primary antibody (1/1000) for 2 hours at 21°C. A biotin conjugated goatanti-rabbit IgG secondary was used at 1/250.
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This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76316).
Intracellular flow cytometric analysis of permeabilized SH-SY5Y cells using ab76316 (red) at 1/20 or a rabbit IgG (ab172730) as a negative control (green). The cells were permeabilized with 2% PFA and a goat anti-rabbit IgG FITC was used as the secondary at 1/150.
实验方案
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (5)
ab219374 被引用在 5 文献中.
- Lee KW et al. Alteration by p11 of mGluR5 localization regulates depression-like behaviors. Mol Psychiatry 20:1546-56 (2015). PubMed: 26370144
- Golubeva AV et al. The mouse cyclophosphamide model of bladder pain syndrome: tissue characterization, immune profiling, and relationship to metabotropic glutamate receptors. Physiol Rep 2:e00260 (2014). WB ; Mouse . PubMed: 24760514
- Ledonne A et al. Neuregulin 1 signalling modulates mGluR1 function in mesencephalic dopaminergic neurons. Mol Psychiatry N/A:N/A (2014). PubMed: 25266126
- Rossi PI et al. Compensatory Molecular and Functional Mechanisms in Nervous System of the Grm1crv4 Mouse Lacking the mGlu1 Receptor: A Model for Motor Coordination Deficits. Cereb Cortex : (2012). WB, ICC/IF ; Mouse . PubMed: 22791805
- Ko SJ et al. PKC phosphorylation regulates mGluR5 trafficking by enhancing binding of Siah-1A. J Neurosci 32:16391-401 (2012). WB . PubMed: 23152621