Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate 5 min in pressure cooker
Sample
Mouse Tissue sections (small intestine)
Specification
small intestine
Permeabilization
No
Fixative
10% Formalin
Other product details
Incubation time
16 hour(s) and 0 minute(s)
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: Dianova anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/250
Additional data
Additional Notes
Very specific staining!
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Thorsten Cramer
Verified customer
提交于 Sep 23 2014