Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (lung)
Specification
lung
Fixative
Formaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Other product details
Dilution
1/100
Incubation time
4 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 1%BSA
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor® 488 Donkey Anti-Rabbit IgG (H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/100
Additional data
Abcam response
Thank you for your valuable feedback. This abreview suggest that this antibody could function in ICC/IF however this application has not been added to the datasheet as we would need further data to confirm this. We welcome other abreviews to determine whether this antibody is suitable for this application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. Yu Fang Wang
Verified customer
提交于 Mar 18 2013