Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Brain slices (Striatum))
Permeabilization
Yes - 15 minutes in PBS-Triton 0.2%
Specification
Brain slices (Striatum)
Blocking step
PBS-Tween 0.05% + Goat Serum 10% + 0.3M Glycine as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative
Paraformaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: DyLight 549
Host species: Goat
Clonality: Polyclonal
Conjugation: DyLight 550
Host species: Goat
Clonality: Polyclonal
Conjugation: DyLight 550
Additional data
Additional Notes
A heat-induced antigen retrieval step was added using Citrate pH 6.0 Buffer (Sigma) in the microwave for 2min30secs at 700W.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jan 23 2018