重组Anti-Mesothelin抗体[SP74] (ab93620)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP74] to Mesothelin
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Mesothelin抗体[SP74]
参阅全部 Mesothelin 一抗 -
描述
兔单克隆抗体[SP74] to Mesothelin -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Pig -
免疫原
Synthetic peptide within Human Mesothelin aa 550 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q13421 -
阳性对照
- IHC-P: Human Mesothelioma tissue. WB: BxPC-3, HeLa, PC3 and A549 cell lysate.
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常规说明
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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纯度
Protein A/G purified -
纯化说明
Purified from TCS by protein A/G. -
克隆
单克隆 -
克隆编号
SP74 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab93620于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.322 µg/ml. Predicted molecular weight: 69 kDa.
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IHC-P | (2) |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
Use a concentration of 0.322 µg/ml. Predicted molecular weight: 69 kDa. |
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Membrane-anchored forms may play a role in cellular adhesion.
Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro. -
组织特异性
Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level). -
疾病相关
Note=Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer. -
序列相似性
Belongs to the mesothelin family. -
翻译后修饰
Both MPF and the cleaved form of mesothelin are N-glycosylated.
Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin. -
细胞定位
Secreted and Cell membrane. Golgi apparatus. - Information by UniProt
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数据库链接
- Entrez Gene: 10232 Human
- Entrez Gene: 100524016 Pig
- Omim: 601051 Human
- SwissProt: Q13421 Human
- Unigene: 408488 Human
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别名
- CAK 1 antibody
- CAK1 antibody
- CAK1 antigen antibody
see all
图片
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All lanes : Anti-Mesothelin antibody [SP74] (ab93620) at 0.322 µg/ml
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate
Lane 2 : BxPC-3 (Human pancreas adenocarcinoma epithelial cell) cell lysate
Lane 3 : PC3 (Human prostate adenocarcinoma epithelial cell) cell lysate
Lane 4 : A549 (Human lung carcinoma epithelial cell) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Predicted band size: 69 kDa
Observed band size: 49,69 kDa why is the actual band size different from the predicted?
Blocking/Diluting buffer and concentration: 5% NFDM /TBST -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human mesothelioma tissue sections labeling Mesothelin with Purified ab93620 at 1/100 dilution (1.4 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Human Mesothelioma stained with Anti-Mesothelin antibody [SP74] (ab93620)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab93620 被引用在 3 文献中.
- Kim H et al. Mesothelin expression and its prognostic role according to microsatellite instability status in colorectal adenocarcinoma. Medicine (Baltimore) 98:e16207 (2019). PubMed: 31261569
- Smith TT et al. Biopolymers codelivering engineered T cells and STING agonists can eliminate heterogeneous tumors. J Clin Invest 127:2176-2191 (2017). PubMed: 28436934
- Sopha SC et al. Diagnostic and therapeutic implications of a novel immunohistochemical panel detecting duodenal mucosal invasion by pancreatic ductal adenocarcinoma. Int J Clin Exp Pathol 6:2476-86 (2013). PubMed: 24228110