Anti-Melanopsin抗体(ab19306)
Key features and details
- Rabbit polyclonal to Melanopsin
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Melanopsin抗体
参阅全部 Melanopsin 一抗 -
描述
兔多克隆抗体to Melanopsin -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Rat Melanopsin aa 1-100.
Database link: Q8R456 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab19306于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 53,85 kDa. Endogenous melanopsin is expressed at low levels making it difficult to detect. We recommend running a positive control and conducting experiments on samples overexpressing melanopsin protein.
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF |
Use a concentration of 10 µg/ml.
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说明 |
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WB
Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 53,85 kDa. Endogenous melanopsin is expressed at low levels making it difficult to detect. We recommend running a positive control and conducting experiments on samples overexpressing melanopsin protein. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 10 µg/ml. |
靶标
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功能
Photoreceptor required for regulation of circadian rhythm. Contributes to pupillar reflex and other non-image forming responses to light. May be able to isomerize covalently bound all-trans retinal back to 11-cis retinal. -
组织特异性
Eye. Expression is restricted within the ganglion and amacrine cell layers of the retina. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. Opsin subfamily. -
细胞定位
Cell membrane. Found in soma, dendrites and proximal part of axons of certain retinal ganglion cells. - Information by UniProt
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数据库链接
- Entrez Gene: 94233 Human
- Entrez Gene: 30044 Mouse
- Entrez Gene: 192223 Rat
- Omim: 606665 Human
- SwissProt: Q14D01 Human
- SwissProt: Q9UHM6 Human
- SwissProt: Q9QXZ9 Mouse
- SwissProt: Q8R456 Rat
see all -
别名
- Melanopsin antibody
- MGC142118 antibody
- MOP antibody
see all
图片
实验方案
数据表及文件
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Datasheet download
文献 (5)
ab19306 被引用在 5 文献中.
- Benedetto MM et al. Non-Visual Photopigments Effects of Constant Light-Emitting Diode Light Exposure on the Inner Retina of Wistar Rats. Front Neurol 8:417 (2017). PubMed: 28871236
- Lu Q & Pan ZH Immunohistochemical Procedures for Characterizing the Retinal Expression Patterns of Cre Driver Mouse Lines. Methods Mol Biol 1642:181-194 (2017). PubMed: 28815501
- Liu M et al. Overexpression of melanopsin in the retina restores visual function in Royal College of Surgeons rats. Mol Med Rep 13:321-6 (2016). PubMed: 26572076
- Jain V et al. Classical Photoreceptors Are Primarily Responsible for the Pupillary Light Reflex in Mouse. PLoS One 11:e0157226 (2016). PubMed: 27295136
- Boudard DL et al. Loss of photic entrainment at low illuminances in rats with acute photoreceptor degeneration. Eur J Neurosci 30:1527-36 (2009). PubMed: 19821841