Application
Western blot
Sample
Mouse Cell lysate - whole cell (HEK293 transfected with mouse GP100 plasmid)
Loading amount
10000 cells
Specification
HEK293 transfected with mouse GP100 plasmid
Treatment
44 hrs post transfection
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Other product details
Dilution
1/250
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: TBS + 0.05% tween 20 + 1% milk
Secondary antibody
Name
Non-Abcam antibody was used: anti-goat IgG HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
TMB
Exposure
30 minute(s) and 0 second(s)
Bands
Specific: 65, 76 kDa
Positive control
293 cells transfected with mouse GP100 expression plasmid
Negative control
Mock transfected 293, 293 transfected with irrelevant plasmid
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Sep 29 2010