Application
ELISA
Sample
Human Recombinant protein (peptide IMDQVPFSV)
Specification
peptide IMDQVPFSV
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
Type
Sandwich (Capture)
Other product details
Incubation time
8 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: NaHCO3 1% PH9,6
Dilution
1/40
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
Detection: Streptavidin HRP 1/5000 in PSB-tween 0,1%, 1h 37°C
Peptide previously biotinylated.
Abtrial code: ABTRIAL-EV7U2
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Maria Machado
Verified customer
提交于 May 12 2015