Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Melanoma cell line WM3248 xenopraph)
Specification
Melanoma cell line WM3248 xenopraph
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric buffer pH6.0
Permeabilization
Yes - 0.1% triton x-100
Blocking step
Dakocytomation x0909 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Other product details
Dilution
1/25
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking solution
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-mouse IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/100
Additional data
Additional Notes
Not all melanoma cell line can be HMB45 positive. I tried 1205Lu cell line but failed even with ABC system. Also not 100% cells are positive due to expression level.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Hongwei Shao
Verified customer
提交于 Feb 25 2010