Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (NB4 cell line(acute promylocytic leukemia))
Specification
NB4 cell line(acute promylocytic leukemia)
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Other product details
Dilution
1/100
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10% normal goat serum in 1X PBS
Secondary antibody
Name
Non-Abcam antibody was used: Cy3-conjugated affinipure Donkey anti-goat
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/200
Additional data
Additional Notes
I tried IF to confirm Mel18 epxression in our system using other company's antibody, it was failed. But i would like to say Mel-18 antibody from Abcam is working well.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Sungsin Jo
Verified customer
提交于 Oct 03 2012