Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (293T transfected with Hu matriptase 2.FLAG)
Total protein in input
1e+007 cells
Specification
293T transfected with Hu matriptase 2.FLAG
Immuno-precipitation step
Protein A
Other product details
Concentration
5 µg/mg lysate
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS
Western Blot antibody
Name
Non-Abcam antibody was used: anti FLAG
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Additional data
Additional Notes
I didn't get the antibody to work well in western blot but it worked very well as an immunoprecipitating antibody. Infact better than the anti FLAG. (Hu matriptase 2 construct was FLAG tagged.)
Lysis buffer was Np40
Lysis buffer was Np40
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. Schimanski Lisa
Verified customer
提交于 Oct 22 2008