Application
Western blot
Sample
Human Cell lysate - whole cell (Human Macrophages Type 2)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
20 µg
Specification
Human Macrophages Type 2
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Odyssey Blocking Buffer
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: IRD 800CW Odyssey
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
Infrared
Exposure
4 minute(s) and 0 second(s)
Bands
Specific: 166 kDa Non-specific: 52, 38, 17 kDa
Positive control
Positive control in this western blot is Macrophages Type 2. These cells express the Mannose Receptor and therefore, are an optimal positive control.
Additional data
Additional Notes
The band of interest can be detected but the antibody is not specific
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Francesco Elia Marino
Verified customer
提交于 Mar 21 2016