Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (muscle)
Specification
muscle
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/200
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Invitrogen alexa fluor anti-rabbit 488
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Abcam response
Thank you for submitting an Abreview and your valuable feedback about the use of ab125028 in IHC-Fr, a application that we has not been validated yet. We welcome any further feedback and Abreviews from other customers who have used this antibody in IHC-Fr which will help us assess how well the antibody works in this application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Thomas Zwakhals
Verified customer
提交于 Mar 15 2016