Application
Western blot
Sample
Cashew nuts (Anacardiaceae) Tissue lysate - whole (whole nut)
Loading amount
50 µg
Specification
whole nut
Treatment
roasted
Gel Running Conditions
Reduced Denaturing (4-20)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 10-12 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Chris Mattison
Verified customer
提交于 Oct 18 2011