Application
Western blot
Sample
Human Cell lysate - whole cell (MCF-7 (breast cancer))
Gel Running Conditions
Reduced Denaturing (8)
Loading amount
20 µg
Specification
MCF-7 (breast cancer)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Milk/TBS-T
Dilution
1/500
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Donkey-anti-rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
45 minute(s) and 0 second(s)
Bands
Specific: 50 kDa
Additional data
Additional Notes
Weak signal
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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提交于 May 21 2013