Application
Western blot
Sample
Rat Tissue lysate - whole (Heart - left ventricle)
Gel Running Conditions
Reduced Denaturing (8)
Loading amount
40 µg
Specification
Heart - left ventricle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBS-T
Dilution
1/500
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Donkey anti-rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 50 kDa Non-specific: 90, 43 kDa
Additional data
Abcam response
Indeed, it seems that this antibody does give additional bands on rat samples. We do not know to what these bands do correspond to. We would to encourage researchers using this antibody to submit also their feedback on the results obtained with in Western blot.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 May 27 2011