重组Anti-MYH2抗体[EPR5280] (ab124937)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5280] to MYH2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-MYH2抗体[EPR5280]
参阅全部 MYH2 一抗 -
描述
兔单克隆抗体[EPR5280] to MYH2 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human MYH2 aa 50-150. The exact sequence is proprietary.
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阳性对照
- Mouse muscle, Rat muscle and Human trachea lysates
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 6.90 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5280 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124937于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/10000 - 1/50000. Detects a band of approximately 220 kDa (predicted molecular weight: 223 kDa).
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说明 |
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WB
1/10000 - 1/50000. Detects a band of approximately 220 kDa (predicted molecular weight: 223 kDa). |
靶标
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功能
Muscle contraction. Required for cytoskeleton organization. -
疾病相关
Defects in MYH2 are the cause of inclusion body myopathy type 3 (IBM3) [MIM:605637]. Hereditary inclusion body myopathies constitute a group of neuromuscular disorders characterized by slowly progressive distal and proximal weakness and a typical muscle pathology including rimmed vacuoles and filamentous inclusions. IBM3 is a variant of hereditary inclusion body myopathies and is characterized by autosomal dominant myopathy with joint contracture, ophthalmoplegia and rimmed vacuoles. Morphological analysis of muscle biopsies from patients indicate that the type 2A fibers frequently were abnormal, whereas other fiber types appeared normal. -
序列相似性
Contains 1 IQ domain.
Contains 1 myosin head-like domain. -
结构域
The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2). -
细胞定位
Cytoplasm > myofibril. Thick filaments of the myofibrils. - Information by UniProt
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数据库链接
- Entrez Gene: 4620 Human
- Entrez Gene: 17882 Mouse
- Entrez Gene: 691644 Rat
- Omim: 160740 Human
- SwissProt: Q9UKX2 Human
- SwissProt: Q5SX41 Mouse
- SwissProt: Q07443 Rat
- Unigene: 667534 Human
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别名
- adult 2 antibody
- Fast 2a myosin heavy chain antibody
- IBM3 antibody
see all
图片
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All lanes : Anti-MYH2 antibody [EPR5280] (ab124937) at 1/10000 dilution
Lane 1 : Mouse muscle tissue lysate
Lane 2 : Rat muscle tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 223 kDa
Observed band size: 220 kDa why is the actual band size different from the predicted? -
Anti-MYH2 antibody [EPR5280] (ab124937) at 1/10000 dilution + Human trachea lysate at 10 µg
Secondary
HRP labelled Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 223 kDa
Observed band size: 220 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab124937 被引用在 7 文献中.
- Zhou X et al. Maternal undernutrition alters the skeletal muscle development and methylation of myogenic factors in goat offspring. Anim Biosci 35:847-857 (2022). PubMed: 34991223
- Zeng X et al. Inhibition of mitochondrial and cytosolic calpain attenuates atrophy in myotubes co-cultured with colon carcinoma cells. Oncol Lett 21:124 (2021). PubMed: 33552245
- Rajasekaran NS et al. Reductive stress impairs myogenic differentiation. Redox Biol 34:101492 (2020). PubMed: 32361680
- Ma X et al. Differential activation of the calpain system involved in individualized adaptation of different fast-twitch muscles in hibernating Daurian ground squirrels. J Appl Physiol (1985) 127:328-341 (2019). PubMed: 31219776
- Wang T et al. Succinate induces skeletal muscle fiber remodeling via SUNCR1 signaling. EMBO Rep 20:e47892 (2019). PubMed: 31318145
- Shahid M et al. Quantitative Proteomic Analysis Reveals Caffeine-Perturbed Proteomic Profiles in Normal Bladder Epithelial Cells. Proteomics 18:e1800190 (2018). PubMed: 30232827
- Zeng X et al. Acylated and unacylated ghrelin inhibit atrophy in myotubes co-cultured with colon carcinoma cells. Oncotarget 8:72872-72885 (2017). PubMed: 29069832