Application
Western blot
Sample
Mouse Cell lysate - whole cell (Muscle cell)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
60 µg
Specification
Muscle cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-T 5% Milk
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Anti-goat peroxidase Conjugate
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
2 minute(s) and 0 second(s)
Positive control
Muscle cell confimed with another MuRF2 antibody (anti-rabbit)
Additional data
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Joao de Oliveira Silvestre
Verified customer
提交于 Feb 11 2016