Application
Western blot
Sample
Mouse Serum (mouse serum)
Loading amount
30 µg
Specification
mouse serum
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Secondary antibody
Name
Abcam antibody:Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP)
Dilution
1/2000
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 135 kDa
Positive control
Ms3 and Ms4 (mouse with pancreatic tumor)
Negative control
Ms1 and Ms2 normal mouse
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 May 25 2012