Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (15% GEL)
Sample
Mouse Cell lysate - whole cell (mouse Embryonic Fibroblasts)
Specification
mouse Embryonic Fibroblasts
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-T
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: GOAT ANTI-MOUSE
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL
Bands
Specific: 48 kDa Non-specific: 48 kDa
Exposure
1 minute(s) and 0 second(s)
Additional data
Additional Notes
Antibody does not detect the right size band corresponding to MTCO2. Further confirmation for nonspecific interaction comes from the inability of antibody to specifically stain mitochondria in immunocytochemistry.
Abcam response
Thank you for your review of this product in mouse. As we have never tested in mouse, we cannot guarantee its use in this species. The homology is pretty good between the immunogen and the mouse protein, so you may wish to try reducing your blocking time to one hour and adjusting the dilution to 1/500 to see if this improves your results in mouse samples.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 May 22 2014