Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Retina)
Permeabilization
Yes - 0.3% Triton
Specification
Retina
Blocking step
5% Donkey Serum, 2% BSA, 0.3% Triton as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Donkey Serum, 2% BSA, 0.3% Triton
Dilution
1/2000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Additional data
Additional Notes
MTCO1 in magenta, nuclear stain in blue (HOESCHT)
Bright band just above the outer nuclear layer corresponds to mitochondria located in the photoreceptor inner segments, confirming the localization specificity of this antibody
Fixed in 4% paraformaldehyde
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Aug 09 2017