Application
ChIP
Sample
Human Cell lysate - nuclear (Jurkat cells)
Negative control
ChIP with rabbit IgG
qPCR on GAPDH promoter
Specification
Jurkat cells
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 8 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Duration of cross-linking step: 8 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Concentration
0.25 µg/µg chromatin
Additional data
Abcam response
This antibody was tested for ChIP using our Abtrial program. The results indicate that this antibody might not be appropriate for ChIP or further optimization might be required. Please contact Scientific Support if you have any questions.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Marion Dubuissez
Verified customer
提交于 Nov 03 2015