Anti-STK3/MST-2 (phospho T180) + MSP/MST1 (phospho T183)抗体(ab79199)
Key features and details
- Rabbit polyclonal to STK3/MST-2 (phospho T180) + MSP/MST1 (phospho T183)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-STK3/MST-2 (phospho T180) + MSP/MST1 (phospho T183)抗体 -
描述
兔多克隆抗体to STK3/MST-2 (phospho T180) + MSP/MST1 (phospho T183) -
宿主
Rabbit -
特异性
ab79199 detects endogenous levels of STK3/MST1 only when phosphorylated at threonine 183 (MST1) and/or threonine T180 (STK3). -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Synthetic peptide (phospho T183). Synthetic phosphopeptide derived from human STK3/MST1 around the phosphorylation site of threonine 183 (R-N-TP-V-I).
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阳性对照
- WB: Extracts from 293 cells treated with H2O2 (100uM, 15mins). IHC-P: Human liver carcinoma tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab79199 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab79199于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 56 kDa.
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IHC-P |
1/50 - 1/100.
|
说明 |
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WB
1/500 - 1/1000. Predicted molecular weight: 56 kDa. |
IHC-P
1/50 - 1/100. |
靶标
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细胞定位
STK3: Cytoplasm. Nucleus. The caspase-cleaved form cycles between nucleus and cytoplasm. MST1: Secreted. -
数据库链接
- Entrez Gene: 4485 Human
- Entrez Gene: 6788 Human
- Entrez Gene: 15235 Mouse
- Entrez Gene: 56274 Mouse
- Omim: 142408 Human
- Omim: 605030 Human
- SwissProt: P26927 Human
- SwissProt: Q13188 Human
see all
图片
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All lanes : Anti-STK3/MST-2 (phospho T180) + MSP/MST1 (phospho T183) antibody (ab79199)
Lane 1 : Extracts from 293 cells, treated
with H2O2 (100uM, 15mins)
Lane 2 : Extracts from 293 cells, treated
with H2O2 (100uM, 15mins) with immunising phosphopeptide
Predicted band size: 56 kDa
Observed band size: 56 kDa -
ab79199, at 1/50 dilution, staining STK3 (phospho T180) + MSP/MST1 (phospho T183) in human liver carcinoma by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising phosphopeptide.
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab79199 被引用在 13 文献中.
- Du T et al. Regulatory effect of microRNA-223-3p on breast cancer cell processes via the Hippo/Yap signaling pathway. Oncol Lett 22:516 (2021). PubMed: 33986876
- Wang W et al. Parthenolide plays a protective role in the liver of mice with metabolic dysfunction-associated fatty liver disease through the activation of the HIPPO pathway. Mol Med Rep 24:N/A (2021). PubMed: 33955510
- Bian SB et al. Leukemia inhibitory factor promotes gastric cancer cell proliferation, migration, and invasion via the LIFR-Hippo-YAP pathway. Ann N Y Acad Sci 1484:74-89 (2021). PubMed: 32827446
- Lupse B et al. Inhibition of PHLPP1/2 phosphatases rescues pancreatic β-cells in diabetes. Cell Rep 36:109490 (2021). PubMed: 34348155
- Li Y et al. Glucocorticoids induce osteonecrosis of the femoral head through the Hippo signaling pathway. Open Life Sci 16:1130-1140 (2021). PubMed: 34746414