Anti-MRas抗体(ab26303)
Key features and details
- Rabbit polyclonal to MRas
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-MRas抗体
参阅全部 MRas 一抗 -
描述
兔多克隆抗体to MRas -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Rat -
免疫原
Synthetic peptide:
KKKKTKWRGDRATGTHKLQ
, corresponding to C terminal amino acids 186-204 of Human MRas -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Constituent: Whole serum -
Concentration information loading...
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纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab26303于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2500. Detects a band of approximately 32 kDa (predicted molecular weight: 24 kDa). Block with 5% milk and dilute in TBST only.
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ICC/IF |
1/1000.
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说明 |
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WB
1/2500. Detects a band of approximately 32 kDa (predicted molecular weight: 24 kDa). Block with 5% milk and dilute in TBST only. |
ICC/IF
1/1000. |
靶标
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功能
May serve as an important signal transducer for a novel upstream stimuli in controlling cell proliferation. Weakly activates the MAP kinase pathway. -
组织特异性
Expression highly restricted to the brain and heart. -
序列相似性
Belongs to the small GTPase superfamily. Ras family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 22808 Human
- Entrez Gene: 25482 Rat
- Omim: 608435 Human
- SwissProt: O14807 Human
- SwissProt: P97538 Rat
- Unigene: 527021 Human
- Unigene: 10641 Rat
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别名
- FLJ42964 antibody
- M ras antibody
- M-ras antibody
see all
图片
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ICC/IF image of ab26303 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26303, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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All lanes : Anti-MRas antibody (ab26303)
Lane 1 : HEK 293T cell lysate
Lane 2 : MCF-7 cell lysate
Lane 3 : HeLa cell lysate
Secondary
All lanes : anti-rabbit HRP conjugated
Developed using the ECL technique.
Predicted band size: 24 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Observed band larger than predicted size, thought to be due to post-translational modifications.
实验方案
数据表及文件
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Datasheet download
文献 (0)
ab26303 尚未被引用在任何文献中。