Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Skin epidermal layer)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer, pH 6.0
Specification
Skin epidermal layer
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: goat-serum contained PBS
Dilution
1/250
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG(H+L)-HRP
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
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提交于 Mar 20 2018