重组Anti-MNK1 (phospho T385)抗体[EPR2370] (ab109102)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2370] to MNK1 (phospho T385)
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-MNK1 (phospho T385)抗体[EPR2370]
参阅全部 MNK1 一抗 -
描述
兔单克隆抗体[EPR2370] to MNK1 (phospho T385) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa cell lysates, treated with Calyculin A; Human colon tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR2370 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109102于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 51 kDa.
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 51 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
May play a role in the response to environmental stress and cytokines. Appears to regulate transcription by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 1 protein kinase domain. -
翻译后修饰
Dual phosphorylation of Thr-250 and Thr-255 activates the kinase. Phosphorylation of Thr-385 activates the kinase. -
细胞定位
Cytoplasm and Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 8569 Human
- Omim: 606724 Human
- SwissProt: Q9BUB5 Human
- Unigene: 371594 Human
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别名
- MAP kinase interacting kinase 1 antibody
- MAP kinase interacting serine/threonine kinase 1 antibody
- MAP kinase signal integrating kinase 1 antibody
see all
图片
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All lanes : Anti-MNK1 (phospho T385) antibody [EPR2370] (ab109102) at 1/1000 dilution
Lane 1 : HeLa cell lysate, untreated
Lane 2 : HeLa cell lysate, treated with Calyculin A.
Lysates/proteins at 10 µg per lane.
Predicted band size: 51 kDa -
Immunohistochemical analysis of MNK1 (phospho T385) expression in paraffin-embedded Human colon tissue using ab109102 at 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab109102 被引用在 4 文献中.
- Zhang Y et al. The SARS-CoV-2 protein ORF3a inhibits fusion of autophagosomes with lysosomes. Cell Discov 7:31 (2021). PubMed: 33947832
- Chen Z et al. MAP kinase-interacting kinase 1 (MNK1) plays as a tumor suppressor in bladder cancer. Transl Cancer Res 9:2588-2598 (2020). PubMed: 35117618
- Zhao Q et al. Novel HSP90-PI3K Dual Inhibitor Suppresses Melanoma Cell Proliferation by Interfering with HSP90-EGFR Interaction and Downstream Signaling Pathways. Int J Mol Sci 21:N/A (2020). PubMed: 32156008
- Attar-Schneider O et al. Migration and epithelial-to-mesenchymal transition of lung cancer can be targeted via translation initiation factors eIF4E and eIF4GI. Lab Invest 96:1004-15 (2016). PubMed: 27501049