Application
Western blot
Sample
Pig Cell lysate - whole cell (Pig cell lystate)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
Pig cell lystate
Blocking step
Milk as blocking agent for 25 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: overnight
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit IgG-HRP Cruz Marker™ compatible:
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 second(s)
Positive control
GAPDH bands were positive.
Additional data
Abcam response
Thank you for participating in our AbTrial program and your valuable feedback. As the antibody had not yet been tested in pig before we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this species. The overall homology of human and porcine MMP9 is 87%. Based on the researcher's result, it seems as if the antibody is not cross-reacting with pig or the cross-reactivity may be batch-dependent.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jun 27 2017