Anti-MMP9抗体[56-2A4] (ab58803)
Key features and details
- Mouse monoclonal [56-2A4] to MMP9
- Suitable for: IHC-P, IHC-Fr, ICC/IF, WB
- Reacts with: Mouse, Rat, Rabbit, Guinea pig, Human
- Isotype: IgG1
概述
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产品名称
Anti-MMP9抗体[56-2A4]
参阅全部 MMP9 一抗 -
描述
小鼠单克隆抗体[56-2A4] to MMP9 -
宿主
Mouse -
特异性
Recognizes the ~92 kDa latent and the ~83 kDa active forms of MMP9. Does not react with MMP1, MMP2, MMP3 or MMP13. The antibody recognizes precursor (92 kDa) and active (83 kDa) forms of human and guinea pig MMP-9. -
经测试应用
适用于: IHC-P, IHC-Fr, ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Rat, Rabbit, Guinea pig, Human -
免疫原
Synthetic peptide corresponding to Human MMP9 aa 626-644.
Sequence:LFSGRRLWRFDVKAQMVDP
Database link: P14780 -
阳性对照
- Breast carcinoma tissue. Conditioned medium of human fibrosarcoma HT1080 cells The protein is secreted so you may need to concentrate the protein from cell culture media. Concentrate protein by TCA, acetone or ammonium sulphate precipitation. HL60 cell lysate, U937 cell lysate, HT1080 cell lysate, human spleen lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.1% Sodium azide
Constituents: 1.64% Sodium phosphate, 0.1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Is purified. -
克隆
单克隆 -
克隆编号
56-2A4 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab58803于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (3) |
Use at an assay dependent concentration.
|
IHC-Fr | (3) |
Use at an assay dependent concentration.
|
ICC/IF | (1) |
Use at an assay dependent concentration. PubMed: 22496779
|
WB | (2) |
Use a concentration of 2 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 78 kDa).
|
说明 |
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IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. PubMed: 22496779 |
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 78 kDa). |
靶标
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功能
May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. -
组织特异性
Produced by normal alveolar macrophages and granulocytes. -
疾病相关
Intervertebral disc disease
Metaphyseal anadysplasia 2 -
序列相似性
Belongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin repeats. -
结构域
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻译后修饰
Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
N- and O-glycosylated. -
细胞定位
Secreted, extracellular space, extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 4318 Human
- Entrez Gene: 17395 Mouse
- Entrez Gene: 81687 Rat
- Omim: 120361 Human
- SwissProt: P14780 Human
- SwissProt: P41245 Mouse
- SwissProt: P41246 Rabbit
- SwissProt: P50282 Rat
see all -
别名
- 82 kDa matrix metalloproteinase-9 antibody
- 92 kDa gelatinase antibody
- 92 kDa type IV collagenase antibody
see all
图片
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (97)
ab58803 被引用在 97 文献中.
- Al-Maawi S et al. Covalent linkage of sulfated hyaluronan to the collagen scaffold Mucograft® enhances scaffold stability and reduces proinflammatory macrophage activation in vivo. Bioact Mater 8:420-434 (2022). PubMed: 34541411
- Huang D et al. Inhibiting effect of miR-29 on proliferation and migration of uterine leiomyoma via the STAT3 signaling pathway. Aging (Albany NY) 14:1307-1320 (2022). PubMed: 35113040
- Park SK et al. Hyaluronic acid hydrogels crosslinked via blue light-induced thiol-ene reaction for the treatment of rat corneal alkali burn. Regen Ther 20:51-60 (2022). PubMed: 35402662
- Hsieh TY et al. Piperine Provides Neuroprotection against Kainic Acid-Induced Neurotoxicity via Maintaining NGF Signalling Pathway. Molecules 27:N/A (2022). PubMed: 35565989
- Zhang Y et al. Pien-Tze-Huang alleviates CCl4-induced liver fibrosis through the inhibition of HSC autophagy and the TGF-β1/Smad2 pathway. Front Pharmacol 13:937484 (2022). PubMed: 36188553