重组Anti-MMP2抗体[EPR1184] - Low endotoxin,Azide free (ab215986)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1184] to MMP2 - Low endotoxin, Azide free
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-MMP2抗体[EPR1184] - Low endotoxin,Azide free
参阅全部 MMP2 一抗 -
描述
兔单克隆抗体[EPR1184] to MMP2 - Low endotoxin,Azide free -
宿主
Rabbit -
特异性
In Western Blot, this product typically gives a weaker signal in some hepatocarcinoma like HepG2, MHCC97L(PMID:33184263 ) et al. Please use reconmended positive control when testing these cells.
Compared with ab92536, ab181286 has higher sensitivity. We recommend ab181286 as an alternative for testing MMP2 in western blot.
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经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IFmore details
不适用于: IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HepG2, L6, Raw264.7 and NIH/3T3 cell lysates; fetal heart and human skin tissue lysate; Human plasma, brain and breast tissue lysate ICC/IF: PC-3 cells Flow Cyt (intra): HeLa and PC-3 cells
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常规说明
ab215986 is the carrier-free version of ab92536.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.20
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR1184 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Alexa Fluor® 488 Anti-MMP2 antibody [EPR1184] (ab237473)
- Alexa Fluor® 647 Anti-MMP2 antibody [EPR1184] (ab237474)
- APC Anti-MMP2 antibody [EPR1184] (ab310884)
- PE Anti-MMP2 antibody [EPR1184] (ab310940)
- Alexa Fluor® 594 Anti-MMP2 antibody [EPR1184] (ab311718)
- Alexa Fluor® 568 Anti-MMP2 antibody [EPR1184] (ab312994)
- Alexa Fluor® 555 Anti-MMP2 antibody [EPR1184] (ab313199)
- Anti-MMP2 antibody [EPR1184] (ab92536)
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab215986于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB | ||
ICC/IF |
Use at an assay dependent concentration.
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说明 |
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Flow Cyt (Intra)
Use at an assay dependent concentration. ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels. -
组织特异性
Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate. -
疾病相关
Defects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes. -
序列相似性
Belongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin-like domains. -
结构域
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻译后修饰
Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3. -
细胞定位
Secreted > extracellular space > extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes. - Information by UniProt
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数据库链接
- Entrez Gene: 4313 Human
- Entrez Gene: 17390 Mouse
- Entrez Gene: 81686 Rat
- Omim: 120360 Human
- SwissProt: P08253 Human
- SwissProt: P33434 Mouse
- SwissProt: P33436 Rat
- Unigene: 513617 Human
see all -
别名
- 72 kDa gelatinase antibody
- 72kD type IV collagenase antibody
- CLG 4 antibody
see all
图片
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All lanes : Anti-MMP2 antibody [EPR1184] (ab92536) at 1/1000 dilution
Lane 1 : Human plasma tissue lysate
Lane 2 : Human brain tissue lysate
Lane 3 : Human breast tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 69,72 kDa why is the actual band size different from the predicted?
Exposure time: 60 secondsThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92536).
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
ab181602 was used as a GAPDH loading control.
Compared with ab92536, ab181286 has higher sensitivity. We recommend ab181286 as an alternative for testing MMP2 in western blot.
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All lanes : Anti-MMP2 antibody [EPR1184] (ab92536) at 1/1000 dilution
Lane 1 : HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysate
Lane 2 : Untreated HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 3 : HepG2 (Human hepatocellular carcinoma epithelial cell) treated with 300ng/ml BFA for 24 hours whole cell lysate
Lane 4 : Huh7 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 5 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 69,72 kDa why is the actual band size different from the predicted?
Exposure time: 60 secondsThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92536).
Blocking and diluting buffer and concentration: 5% NFDM /TBST.
ab181602 was used as GAPDH loading control.
Compared with ab92536, ab181286 has higher sensitivity. We recommend ab181286 as an alternative for testing MMP2 in western blot.
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Intracellular Flow Cytometry analysis of PC-3 (human prostate adenocarcinoma) cells labeling MMP2 with purified ab92536 at 1/180 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92536).
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Overlay histogram showing HeLa cells fixed in 4% PFA and stained with purified ab92536 at a dilution of 1 in 400 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92536).
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Immunofluorescence staining of PC-3 cells with purified ab92536 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab92536 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92536).
实验方案
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (24)
ab215986 被引用在 24 文献中.
- Xu Z et al. MicroRNA MiR-490-5p suppresses pancreatic cancer through regulating epithelial-mesenchymal transition via targeting MAGI2 antisense RNA 3. Bioengineered 13:2673-2685 (2022). PubMed: 35043728
- Zhou M et al. LINC01433 targets miR-506-3p to promote the biological progress of nasopharyngeal carcinoma cells. Eur Arch Otorhinolaryngol 278:3363-3374 (2021). PubMed: 33479848
- Hong X et al. Biological effects of NODAL on endometrial cancer cells and its underlying mechanisms. Exp Ther Med 21:402 (2021). PubMed: 33717261
- Zhan X et al. Sevoflurane inhibits cell proliferation and migration of glioma by targeting the miR-27b/VEGF axis. Mol Med Rep 23:N/A (2021). PubMed: 33786635
- Guo J et al. SPRY4 suppresses proliferation and induces apoptosis of colorectal cancer cells by repressing oncogene EZH2. Aging (Albany NY) 13:11665-11677 (2021). PubMed: 33879635