Application
Western blot
Sample
Human Tissue lysate - whole (Heart, Left Ventricle)
Loading amount
40 µg
Specification
Heart, Left Ventricle
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/500
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Secondary antibody
Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 66 kDa
Additional data
Additional Notes
Lanes 1,6 and 11 were loaded with Bio-Rad MW Marker, the rest were loaded with human left ventricular lysates.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dawn Bowles
Verified customer
提交于 Dec 28 2012