Application
Flow Cytometry
Sample
Human Cell (Ovarian Cancer Cell Line TOV21G)
Permeabilization
No
Gating Strategy
live cells
Specification
Ovarian Cancer Cell Line TOV21G
Preparation
Cell harvesting/tissue preparation method: Typsin
Sample buffer: 1% BSA in PBS with 0.05% sodium azide
Sample buffer: 1% BSA in PBS with 0.05% sodium azide
Fixation
no fixation
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Diluent: on ice
Dilution
1/100
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Alexa Fluro 647
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Additional data
Additional Notes
As shown on the graph, the red peak stands for control cells stained with Rabbit IgG isotope and the blue peak represents cells stained with MICA antibody (ab62540). More than 10,000 cells are tested in this experiments for each sample.
I also used this antibody for western blot. There's a specific band comes up around 46 kd, however, there're quite a lot non-specific bands on the lot as well.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jun 15 2015